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We want to talk about how the product can get contaminated with mold and yeast before we describe the lab test technique. This may occur as a result of the drying procedure, in which farmers spread out the leaves to dry in the sun. Typically, bulk orders for marine containers are filled by drying like this.
This Drying is a mass production technique that is frequently used to meet bulk orders for sea containers based on quantity and low production costs. Once the leaves have been declared dry, they are taken to be crushed by hand or machine, but any remaining wet veins will mix in with the dried leaf crumbs. After a month, the color of the powdered or crushed leaves will start to alter and begin to smell musty. Because the leaves are dried in the sun for the majority of the day or more during the rainy season and in an open area, anything can contaminate the leaves throughout the drying process, making this approach the least hygienic. Products produced using this method cannot be stored for longer than two months. Gamma and lab tests could not fix this mistake.
Given that the lab test results from a 10-gram sample cannot accurately represent the 1000 kilograms more batch you ordered and that the lab test is very simple to manipulate by only choosing a robust and hygienic batch to test, it is crucial for you to understand how your product should be produced by your supplier. To do this, look for references in articles about how hygienic products are produced and ask your potential supplier about this. Watch this video; there are many good explanations for your reference. ( https://youtu.be/czxkWLyUiPc )
Knowing how potent a Kratom batch is might be one of the most interesting information for a Kratom user. Also, to categorize different product qualities and to apply a fair pricing strategy, the information about alkaloid contents is crucial.
We test for Kratom’s primary alkaloids Mitragynine and 7-Hydroxymitragynine.
The usual range in raw plant material of Kratom is between 1,2%-1,8% Mitragynine. Results over 2,3% are extremely rare and often not realistic.
The basic heavy metals that we have our Kratom tested for are:
Products can only to human consumption of all tested heavy metals are within the quantitation limit.
By running this test different possible microbiological contaminations are checked. In detail test for:
Salmonella
Listeria monocytogenes
Yeasts and Molds
Coliform bacteria
E. coli Bacteria
Especially Salmonella are a serious risk for Kratom users and should absolutely be tested. In 2018-2019 there was a recall in 41 US-States after 199 cases of salmonellosis were linked to the consumption of Kratom products. By using a Polymerase Chain Reaction to detect Salmonella in Kratom products can make sure that it detects a contamination even if there is just 1 Salmonella cell in the sample that is tested. In this case the whole batch is considered unqualified for sale and is discarded.
After having tried different Kratom-specialized testing laboratories and sending samples from the same batch to different labs, you will recognize that test results differ strongly between certain laboratories and other labs. This can occur due to slightly inaccurate testing processes. There are numerous points of error that can occur in any analysis. Common ones include weighing errors, dilution errors, instrument setup errors, and calculation errors. There are also numerous alkaloid compounds that can co-elute with mitragynine, especially as an HPLC column degrades. Differences in mobile phase preparation can also significantly affect the separation. This could also be caused by standard degradation.
Therefore, it is important to choose the right laboratory to have the most accurate results and not the highest alkaloid content since there are peaks that could mix up different alkaloids into one.
Trusted laboratory uses the same quality system for the assay, as they use all their ISO-certified methods in order to guarantee accurate results. This includes (but is not limited to) calibration and maintenance of equipment, method controls, documentation, review, and retention of all raw data.
The matrix of alkaloids is very complex, so without a good HPLC separation, you can have co-eluting peaks that could be mistakenly labeled as Mitragynine or 7-Hydroxymitragynine. There are also a number of peaks near the Mitragynine peak that can get labeled in with it, resulting in a high bias (Paynantheine is the big one).
Trusted laboratory must optimized their separation to remove this peak from their calculation of mitragynine. In addition, this analysis yields some long-term memory effects on the HPLC system that reduce the resolving power over time. It can also affect standard responses negatively, which will increase sample concentrations.
The lab we are working with has taken steps to ensure their instruments remain clean and the separation remains consistent from run to run. Also, they monitor their standard response from run to run.
They record all data related to standard preparation, sample preparation, calculations, etc. Their calculation spreadsheets are locked in order to prevent mistakes, and this spreadsheet automatically transfers results to their reporting software to minimize transcription errors.
They have a control sample analyzed with each run as well as standard brackets to ensure the calibration remains consistent throughout the analysis. All manual transcriptions are checked by a second analyst.
Note!
Our product lab test results will be published here as soon as possible.
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